
Starch
Chemistry:
Starch consists
of two glucose polymers: amylose and amylopectin. Although
both polymers are composed of D-glucopyranose molecules,dissimilarities
between the two polymers result in major differences in characteristics.
Amylose
and amylopectin do not exit free in nature,but as components
of the starch granules.The size,shape and structure of the
granules vary substantially among botanical source.
| Characteristic |
Amylose |
Amylopectin |
| Shape |
essential linear |
branched |
| Linkage |
a-1,
4(some a-1, 6) |
a-1,4 and a-1,6 |
| Molecular weight |
<0,5 million |
50-500 million |
| Filmforming |
strong |
weak |
| Gelling |
firm |
soft |
| Colour with Iodine |
blue |
reddish brown |
| Starch |
Amylose (%) |
Amylopectin |
| Maize (corn) |
25 |
75 |
| Waxy
maize |
1-5 |
95-99 |
| Wheat |
25 |
75 |
| Potato |
20 |
80 |
| Tapioca |
17 |
83 |
| High
Amylose maize |
50-70 |
30-50 |
IS there
a way to determine the starch concentration in a solution
using simple apparatus?
Yes, there
is a way to determine the starch concentration in a solution
(or, probably more usually in a suspension) but if you want
to do it you will need to carry out a few experiments to
perfect your method. Iodine solution, usually available
in any school lab will turn starch a deep shade of blue.
If you were to use iodine solution and drop it into samples
containing different amounts of starch the more concentrated
samples containing samples would turn a darker blue than
the more dilute samples. The differences in blueness could
be detected using a simple photometer (usually found in
schools).To be able to test an unknown sample you would
need to construct your own "calibration curve". A suggestion
of how to start of is as follows:
1.Make up
a batch of iodine solution so that you can use the same
batch for all your tests.
2.Make up at least 10 dilutions of a concentrated starch
suspension. Make this suspension by using a carefully weighed
out amount of starch(you will probably need to order this
from your school supplier if you don't have any)in a set
volume of water-say 10ml.Dilute one in ten, one in ten etc
until you have 10 samples, all 10 ml.
3.Add a small amount of iodine solution to each sample(one
drop should do).
4.Measure
each sample, together with plain water in the photometer
and plot your readings(minus the reading for the water on
its own)against concentration of starch. Construct a curve
from your points.
5.Take 10ml of your unknown suspension and add 1 drop of
iodine solution. Measure in the photometer and compare the
reading you get with your curve. You should be able to estimate
the amount of starch in your unknown sample.
Starch
Granules
Starch is stored in plants in the form of granules. The
granules first appear in the plant cell as minute points,
growing rapidly to fill the cell. The granule is made up
of stratifies layers of starch formed around the nucleus
called a hilum .As these layers develop, the starch granule
assumes a size and shape characteristic of the specific
plant.
Tapioca
starch granules vary in diameter form 5-35 microns, potato
starch from 15-100 microns, maize from 5-25 microns, while
rice starch granules are only about 3-8 microns in diameter.
The shapes vary from near perfect spheres to flattened ovoids,
elongated disks, polygons and many others. By observing
the size and shape of the granule structure is , an experience
individual can identify the plant source of a starch even
in mixtures of dry starch. Variations in starch granules
from different plants may be microscopically.
The granule structure is not just a loose agglomeration
of glucose polymers. It is systematically structured with
the starch molecules oriented in specific spherocrystalline
patterns. If the starch granules in aqueous suspension are
microscopically observed in polarized light, a typical Maltese
cross pattern is observed.
Starch granules
are essentially insoluble in cold water. Their unique functional
characteristics are the result of three major changes they
undergo when heated with water: gelatinisation (viscosity
increase), pasting and retro gradation. Retro gradation
is especially evident when amylose containing starches are
cooled, resulting in water release(syneresis).